Confocal Single-Molecule FRET Microscope - CAD graphic


We are an interdisciplinary lab based in the Department of Chemistry at the University of Sheffield.

Research in the Craggs Lab involves the development and application of single-molecule fluorescence techniques to addressing crucial questions across physics, chemistry and the life sciences.

We have used single-molecule Forster Resonance Energy Transfer (smFRET – a molecular ruler for the 30-90 Å scale) to address questions of protein folding, and DNA transcription, replication and repair. These methods are capable of observing individual molecules and molecular interactions in real time, and understanding their dynamics.

In addition to this mechanistic work, we have shown we can use smFRET to measure absolute distances with angstrom accuracy, opening the door to FRET driven structural biology.